where to start? 1st what is the resolution and Rfree of your model? Have you cut the model down to 1 molecule in the ASU, and what does Matthew's predict for your crystals as far as number in ASU? If all of those answers are reasonable, floppy regions can often cause failure in MR. I would cut 15-20 residues of the N and C terminus and try in phaser and Molrep again.
Kevin P Madauss V-161a US Structural Biology GlaxoSmithKline Office: 919.483.3759 5 Moore Drive Cell: 919.924.6436 RTP, NC 27709 "Meetmr Ss" <meetm...@yahoo.com> Sent by: "CCP4 bulletin board" <CCP4BB@JISCMAIL.AC.UK> 24-Dec-2008 02:07 Please respond to "Meetmr Ss" <meetm...@yahoo.com> To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] MR- Problem-76 % seqeunce identity-no solution Dear all, I struck with MR problem. MY target has 76% sequence identity with the model. I tried Phaser, AMoRe and Molrep. None of them gave me satisfactory solution. If you have any suggestions and or New programs I would like to try. Thanks somu
