You can also try TALON cobalt affinity resin from Clontech ( http://www.clontech.com/products/detail.asp?product_id=10588&tabno=2) or the high yield PrepEase resin from USB ( http://www.usbweb.com/category.asp?special=&cat=234&id=78806).
On Tue, Jan 27, 2009 at 4:34 PM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > Hi Fred, > > I used to worked with a protein which would not bind to the Qiagen > NiNTA (really *NOT*) but beautifully to IDA resin (e.g. Amersham > Pharmacia). > > This was under non-denaturing conditions, though, and I don't know whether > this applies to the denatured state. It may be worth a try. > > Tim > > -- > Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > > > On Tue, 27 Jan 2009, Fred wrote: > > Hi ccp4 list, >> I am trying to purify a his-tag protein by metal affinity chromatography. >> The protein was expressed in inclusion bodies and its his-tag doesn't bind >> the Qiagen Ni resin in denatured conditions (urea 8M or GndHCl 6M). Playing >> with NaCl and detergents didn't help much. >> Any help is appreciated. >> Fred >> > -- Jim Fairman Graduate Research Assistant Department of Biochemistry, Cellular, and Molecular Biology (BCMB) University of Tennessee -- Knoxville 216-368-3337 jfair...@utk.edu james.fair...@case.edu
