Rana, to a large extent this depends on what you what to do with the delta-F's afterwards (you didn't say). If all you want to do is calculate a map, Patterson or Fourier, (which is probably what we do most of the time with delta-F's) then you don't need to calculate the difference as a separate step, the FFT input routine will do it for you, also applying an appropriate phase if required. Or even if you want to say calculate normalised moduli of the differences for direct methods, again the ECALC program will calculate the difference before doing the normalisation. It goes without saying that subtracting F's is pretty meaningless if they are not on the same scale, and any relative B factor has been taken out as well.
-- Ian > -----Original Message----- > From: owner-ccp...@jiscmail.ac.uk > [mailto:owner-ccp...@jiscmail.ac.uk] On Behalf Of Rana Refaey > Sent: 29 January 2009 14:36 > To: ccp4bb@jiscmail.ac.uk > Subject: Fobs - Fobs > > Hi, > > I was wondering if anyone knows what programme I need to use > to subtract the Fobs of two different crystals from each other. > > Regards, > Rana > > > ________________________________ > > Invite your mail contacts to join your friends list with > Windows Live Spaces. It's easy! Try it! > <http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url =/friends.aspx&mkt=en-us> > Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing i.tic...@astex-therapeutics.com and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof. Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, Cambridge CB4 0QA under number 3751674
