Hi,
I wouldn't bother either. I once phased a structure based on about 160
oxidized Se in the AU (Hothorn et al., JBC, 2006). Just make sure that
most of them are oxidized and do a proper absorption scan.
best
Michael
aka akaka wrote:
Dear All
I would like to know whether oxidation of Se entails any problem for
SAD or MAD experiments and/ or how to resolve it. Cannot use DTT or
reducing agents in my protein (extracellular and disulphide bonds are
important).
Thanks
Dr. R.Depetris
Weill Cornell Medical College
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