I have purified my protein granulocyte colony stimulating factor using chromatography steps. my protein is relatively pure when analysed by reducing and non-reducing SDS PAGE method. however non-reducing page shows DIMER presence. i have about 250 ml pure protein sample and do not want to perform gel permeation chromatography as it will be time consuming. is there a way to get rid of dimers by some other method. can dialysis help me. kindly guide me as i have to formulate the product and fill it quickly.
Thanks in anticipation. meg
