Hello Jerry,
We use the Van Duyne et al feedback inhibition method with BL21
and have never had a problem.
ho
UC Berkeley
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Date: Thu, 26 Mar 2009 14:58:57 -0700
From: Jerry McCully <for-crystallizai...@hotmail.com>
Subject: selenomethionine labeling
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Dear All:
I got a problem with the expression of one N-terminal His6 tagged pro=
tein in B834 cells when I tried to do selenomethionine labeling.=20
However=2C the protein can be expressed in BL21{DE3) cells.=20
Welcome any troubleshooting tips.
All the best=2C
Jerry
