Hello Jerry, We use the Van Duyne et al feedback inhibition method with BL21 and have never had a problem.
ho UC Berkeley ------------------------------ Date: Thu, 26 Mar 2009 14:58:57 -0700 From: Jerry McCully <for-crystallizai...@hotmail.com> Subject: selenomethionine labeling --_6abd9eb3-ba7b-436a-99d3-053f7f3a6771_ Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Dear All: I got a problem with the expression of one N-terminal His6 tagged pro= tein in B834 cells when I tried to do selenomethionine labeling.=20 However=2C the protein can be expressed in BL21{DE3) cells.=20 Welcome any troubleshooting tips. All the best=2C Jerry