Liew Chong Wai wrote:
Hi,
Good day
I am currently building my structure by using COOT. My protein is a
tetramer protein and I have fit my protein sequence into one of the
monomer of the homologous model. May I know how can I replace
other monomer with the amended monomer??
Thanks
*Chong-wai*
Hi Chong-wai,
Several possibilities to generate the tetramer from the monomer, let say A.
You can calculate the RT matrices ( in CCP4 or using LSQMAN and MOLEMAN2
or using O for example) to superpose the monomer A onto B, C and D and
then apply these matrices to the amended monomer (A) in order to
generate the tetramer.
You can also superpose the monomer A onto B in COOT by using SSM or LSQ
and save the new position as monomer B in COOT.... etc... and then you
can generate 4 monomers and the tetramer. Do not forget to change the
chain ID before refinement.
Best regards,
David
--
David Cobessi
Institut de Biologie Structurale
41, Rue Jules Horowitz
38027 Grenoble Cedex-1, France
Tel:33(0)438789613
33(0)608164340
Fax:33(0)438785122
