Matt, Your best bet would be to purchase the actual well condition from the manufacturer. I know that both Hampton and Emerald can sell you a larger quantity of what comes premixed in the screen. I am sure other manufacturers can do the same. If you are lucky they might be able to match the lot or batch number as well. You can also purchase the individual reagents (including the glycerol, buffer, etc.) direct from the manufacturer and use these to screen around your conditions. That way you will know that you are using the exact same components all the way around. I have seen cases where a protein will crystallize in the condition purchased from the manufacturer, but not in one made up in the lab, so it is worth a try.
Hope this helps, Gene ======================================= Eugene Masters, Ph.D. mast...@southernresearch.org Postdoctoral Researcher Protein Crystallography Southern Research Institute 2000 Ninth Avenue South Birmingham, AL 35205 Office: 205- 581-2244 Fax: 205-581-2093 ======================================= Please see www.southernresearch.org for information about our capabilities. Southern Research Institute is affiliated with the University of Alabama at Birmingham. Confidentiality Notice The information contained in this communication and its attachments is intended only for the use of the individual to whom it is addressed and may contain information that is legally privileged, confidential, or exempt from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify mailto:postmas...@southernresearch.org (205) 581-2999, and delete the communication without retaining any copies. Thank you. -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Matthew Alan Bratkowski Sent: Wednesday, April 01, 2009 9:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Glycerol for crystal screens Hi. I am trying to optimize a commercial crystallization condition that contains glycerol. So far, I have not been able to even reproduce the results from the initial screen, and I thought that this may be due to poor quality glycerol (among other possibilities). I believe that the glycerol that I'm using is reagent grade and am not sure what company it is manufactored by. Can anyone recommend a supplier and grade of glycerol suitable for making crystallization screens? Thanks, Matt
