Dear Laurie, On Thu, May 07, 2009 at 04:29:40PM -0400, Laurie Betts wrote: > Specifically, hemoglobin, in original PHASER (unrefined) solution DELFWT > an continuing to show up in refined > DELFWT coefficients. But I have seen it in other cases too. > > What does it mean?
It tells you to 'take something out' of your model. And your model consists of the atoms you placed (PDB file) and the bulk solvent. Since there aren't any atoms there I would guess it is the bulk-solvent masking that is wrong there: it puts bulk solvent in places where there should be nothing. So you need to tell the program to include this region into the mask used for the bulk solvent correction. Cheers Clemens > > Thanks > > > Laurie Betts > University of Pittsburgh > X-ray Crystallography Facility Manager > Department of Structural Biology > 3501 Fifth Avenue, > Pittsburgh, PA 15260 > 412-383-5839 > lab...@pitt.edu -- *************************************************************** * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * * Global Phasing Ltd. * Sheraton House, Castle Park * Cambridge CB3 0AX, UK *-------------------------------------------------------------- * BUSTER Development Group (http://www.globalphasing.com) ***************************************************************
