Dear All
I got recently a comment on a paper review suggesting me to perform CD
spectroscopy to determine the alpha-helical content of a mutant protein
involved in a human disease. The diferences between the wild type and
the mutant protein are just 17 aminoacids. My question is if CD
spectroscopy could detect secondary structure differences between the
wild-type and the mutant proteins based on this aminoacid difference.
Let's say, is it possible to detect by CD the presence or absence of a
17 aa helix in a protein of 400 aa.
I would appreciate your opinion
All the best
Francisco J. Enguita
ITQB
Oeiras
Portugal
