Hi all,
Maybe this is more of a statistics/normalization question, but say you
have the same molecule crystallized in two different states. How would
you put their refined b-factors (directly from the pdb) on the same
scale and say compare the b-factors of residues in the binding pocket?
I figure some normalization scheme is involved, but are there any
specific examples ? (quantile normalization? z-score?)
Are there any papers that discuss interpretation of b-factors (being a
crystallographic property) to the relative dynamics/flexibility of the
residue in the crystal (and subsequently in solution if the residue is
not a crystal contact?). I'm particularly interested in the dangers of
such an interpretation.
Thanks!
FR
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Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
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8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
