Hey,I have a GST fusion protein secreted into express5 (with hint of serum), it does not bind beads. Changing pH with 4xPBS limits ppt and enables binding but in a scale up I think it makes sense to use a TFF/CFF setup for concentration and diafiltration.
Does anybody have advice about purifying proteins from insect media? Is TFF the right choice? And any recommendations for TFF system/brand will be appreciated.
Cheers, Mark
