You seem to be describing the MCS found in many TEV-site-containing
expression plasmids (am I missing something?) E.g., look at the
sequences in Sheffield et al., Protein Expression and Purification
15, 34 –39 (1999) (let me know if you want a PDF, I don't want to
send it to the whole bb)
On 5 Jun 2009, at 5:41 PM, Jacob Keller wrote:
Dear Crystallographers,
Does anybody have a TEV-protease-site-coding nucleotide sequence
with a commonly-used restriction site in it, preferably right at
the end? Alternatively, does some somebody know of a program to
determine all equivalent codon permutations for a small coding
region, filtered for resulting restriction site possibilities? It
seems like it would be an easy enough script to write...
(I have already done some googling around for such a program, with
not much luck.)
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
(215) 762-7706
pat.l...@drexelmed.edu
