we have excellent experiences with cocoamidopropylbetaine (CAPB)
especially in SAXS experiments of protein-protein and protein-rna complexes,
js
At Thu, 9 Jul 2009 17:57:36 -0700, Susan Tsutakawa wrote:
Hi Bill,
5-10% glycerol usually helps in the majority of cases. However, some
proteins require a scan of different conditions and different protein
concentrations. Like everything else, it's protein dependent.
Changing the wavelength can also make a difference. I also know some
SAXS beamlines like at the APS have capillary flow cells so they just
do hit and runs.
Susan
On Jul 9, 2009, at 4:45 PM, William Scott wrote:
Hi folks:
A colleague of mine here is doing small angle X-ray scattering at
SSRL and finding his samples are suffering significant radiation
damage. He asked me for advice as to what potential radical
scavengers might be useful. I told him to take 10 mg of ascorbic
acid and to call me in the morning.
Anyone have a better suggestion that I might be able to pass along to him?
Many thanks in advance.
Bill
