Sebastiano-- http://www.sbg.bio.ic.ac.uk/had/ good table of HA and pH ranges
http://www.shapirolab.org/lab-links/had.html links to a variety of HA databases HTH! annie Annie Hassell Glaxo Smithkline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) annie.m.hass...@gsk.com "Miguel Ortiz Lombardia" <miguel.ortiz-lombar...@afmb.univ-mrs.fr> Sent by: "CCP4 bulletin board" <CCP4BB@JISCMAIL.AC.UK> 15-Jul-2009 09:21 Please respond to "Miguel Ortiz Lombardia" <miguel.ortiz-lombar...@afmb.univ-mrs.fr> To CCP4BB@JISCMAIL.AC.UK cc Subject Re: [ccp4bb] heavy atom derivative choice Hi Sebastiano, Have a look at HATODAS: http://hatodas.harima.riken.go.jp/ Good luck, Miguel Le 15 juil. 09 à 14:33, Sebastiano Pasqualato a écrit : > Hi all, > I've got crystals of a protein of ca 200 residues, with 2 free > cysteines, 5 histidines, 2 methionines. > We have nice diffraction for the native crystals, that grow in 150 > mM KSCN, 17% PEG 3350, bis tris propane pH 8.8. > We are crystallising the SeMet derivative, but I'm not completely > sure I will be able to have nice crystals by saturday, when we have > tunable time at the ESRF. > I was thinking of trying with some heavy atom soaks, but only have > like 30 crystals, so limited trials allowed! > Which compound would you advice as more likely to work, and thus > worth testing? > Thanks in advance for the suggestions, > ciao > s > > > > -- > Sebastiano Pasqualato, PhD > IFOM-IEO Campus > Dipartimento di Oncologia Sperimentale > Istituto Europeo di Oncologia > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5094 > > -- > This message has been scanned for viruses and > dangerous content by MailScanner, and is > believed to be clean. > -- Miguel Ortiz Lombardía Architecture et Fonction des Macromolécules Biologiques (UMR6098) CNRS, Universités d'Aix-Marseille I & II Case 932 163 Avenue de Luminy 13288 Marseille cedex 9 France Tel : +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 e-mail: miguel.ortiz-lombar...@afmb.univ-mrs.fr Web: http://www.pangea.org/mol/spip.php?rubrique2 [attachment "emfalert.txt" deleted by Annie M Hassell/PharmRD/GSK]
