>For gel-shift assay, do people normally use a special gel tank for heavy >metal work?
We used to use a Pharmacia Phast system, as this is bufferless and made it very easy to contain heavy metal contamination. Ours caught fire and died a couple of years ago, and I have yet to find a good cheap replacement - does anyone know of a native gel equivalent of the Invitrogen E-PAGE for example? In the absence of such niceties, just treat the gels and electrophoresis solutions as heavy metal waste.