Hi all,
I am doing initial crystallization screening for a membrane protein purified in DDM. The actual concentration of DDM in the protein solution is >> 2 CMC after the protein concentration step (Amicon MWCO 50 kD used). I just found over 50 conditions out of 1000 give me crystals. Having worked on soluble proteins, frankly they all look ugly to me to different degrees. I have selected a few conditions to go after, hoping that they will grow big enough for me to verify the crystal identity by gels. I wonder if there is a quick way to tell if they are detergent crystals. I know a microscope with UV capacity would do the job, but we probably will not get one very soon. I have read through all threads on CCP4BBS regarding detergent crystals. I have an impression that it is actually not easy to crystallize detergents--please correct me if I am wrong. Your thoughts and comments are helpful. Thank you for your attention. -- Best regards, Joe
