Dear Chris, The answer depends on why you want to exclude oxygen for crystallisation and how many screens you want to do. Did you purify your protein in the absence of oxygen? If you only want to set up a few screens for a single construct - I would suggest you put the screens and the plates in your anaerobic chamber a week before you want to use them. Then you set up the drops manually either in linbro plates or in 96 stripwell plates that you can seal 8 reservoirs at a time. Depending on the size of your anaerobic chamber you can also put a microscope in there to look at the drops (otherwise I would suggest you get plexiglass boxes that you can close tight to take the plates out for inspection). I have never tried but it should also be posssible to pipett the drops with an 8-channel pipett with some practice. You could prefill and seal the plates outside bring them into the chamber let them equilibrate for a week and then set up the drops. I personally think that the Quiagen plates are quite large and you usually don't have too much storage space in the anaerobic chamber.
Best regards, Alexander -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Christopher Rife Sent: Wednesday, September 02, 2009 7:34 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] anaerobic crystallization Hi, Does anyone have tips or suggestions for getting started with anaerobic crystallization? Searching through the archives, I was able to find some discussion about various glove box options (http://tinyurl.com/ccp4-glovebox), but not about the process itself (we already have a box). To simplify things, would it be worthwhile to perform the initial screens in something like the pre-filled Qiagen screens (http://tinyurl.com/qiagen-xseal)? Do I need to worry about evaporation while solutions are being degassed (esp volatiles)? Better/easier to try microbatch? Thanks for any input. Chris ________________________________________________________________________ ________ ___________________ This is an e-mail from Danisco and may contain confidential information. If you are not the intended recipient and you receive this e-mail by mistake, you are not allowed to use the information, to copy it or distribute it further. Please notify us and return it to Danisco by e-mail and delete all attachments. Thank you for your assistance. ________________________________________________________________________ ________ __________________
