Take two siliconised glass cover slips. Place 2-5ul of mother liquor on one and transfer a single crystal to this solution. Place the other cover slip on top and press down. The two optically flat surfaces crush the crystal (best observed using a microscope). Slide the two cover slips apart and use 25-50ul of mother liquor to hoover up the carnage. This is your seed stock. Use neat or up to x10,000 dilution. Freezing this stock can increase its potency. Try more ore less grinding for bigger or smaller seed fragments.
David -------------------------------------------------------------------------- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 15 Stanhope Gate, London W1K 1LN. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies. -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of Ed Pozharski Sent: 14 September 2009 17:47 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Preparation of seed-stocks without seed-beads You can use the glass cell disruption pestle http://www.vwrsp.com/catjpg/mp0/mp0440.jpg to grind them up. They cost money, of course, and if you plan to buy something, why not go for seed bead kit? But if you want something very cheap, then you'll find this amazing discovery interesting: "Outer diameter of the bottom of a 0.5 mL "eppendorf" tube is the same as the inner diameter of a 1.5 mL "eppendorf" tube." This is known as "eppendorf mortar-pestle phenomenon" :) On Sat, 2009-09-12 at 13:52 +0530, james09 pruza wrote: > Dear CCP4bbers, > > Can anyone suggests how to make seed-stocks if one is not having > seed-beads... Is there any other methods to crush the crystals for the > same purpose. What if it is simple vortexed. Off-course there wold be > all sorts of sizes, the intact crystals as well. > Please suggest. > > Thanks a lot more for previous help. > James. --
