Dear crystallographers, Sorry for the non-ccp4 query. I am new to this field and need some suggestions. My question is, why some protein takes longer time to crystallize, say 6-8 months, and it is the only condition to get the crystals.? What are the ways to get the crystals faster.
The crystal appears with 60% of 2-Methyl, 2-4 Pentane Diol and only at 4 degree with very low concentration of NaCl. I have got some of the sugggestions earlier from the CCP4-discussion board for microseeding, but it did not work. All suggestions from the experts are welcome. Thanks. James
