Only easy if you happen to have silica gel TLC plates and
a chromatography jar lying around, perhaps from some
phospholipid analysis:
A strategy for identification and quantification of
detergents frequently used in the purification of membrane proteins
Laura R. Eriks, June A. Mayor, and Ronald S. Kaplan
Analytical Biochemistry 323 (2003) 234–241
This paper recommends spotting on a TLC plate and running
beside standard amounts of the same detergent. From intensity/size
of the detergent spot after developing you can bracket the detergent
concentration. (And by the way they found that detergents are concentrated by
ultrafiltration). To increase sensitivity, speedvac a volume too large to
spot on the plate, dissolve the residue in Me0H.
Ed
wei...@crystal.harvard.edu wrote:
Hi Folks:
After concentrating a membrane protein, is there a (easy) way of measuring
the detergent concentration in the sample?
Regards,
Weikai
