Dear Colleagues,
In the proper buffer (e.g. PBS) and after sterile filtration
(possibly, also upon addition of 0.02 % NaN3) Fab fragments can be
stored at 4 oC and stay functional over more than a year.
For long term storage they should be shock-frozen in liquid nitrogen
and may be kept at -20 or -80 oC. As for any protein, repeated
freeze-thawing should be avoided.
Arne Skerra
At 4:02 Uhr -0500 13.11.2009, Anna Gardberg wrote:
Hello Jan,
I stored intact IgGs as an ammonium sulfate cut at 4C with a little
azide for several months with no problems, but I can't recall ever
trying long-term storage of the Fabs. Good luck.
-Anna
On Fri, Nov 6, 2009 at 8:52 AM, Jan Rash <jan...@googlemail.com> wrote:
Dear All,
Does any body have any clue about the stability of monoclonal Fab fragments.
How long can they survive at 4 degree? What is an ideal temperature for
their storage; 4 degree, -20 or -80.
thanks a lot
> Jan
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Prof. Dr. Arne Skerra ske...@wzw.tum.de
Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351
Technische Universitaet Muenchen Fax: -4352
85350 Freising-Weihenstephan
Germany http://www.wzw.tum.de/bc
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