Marie-Helene-- We have included 3-5% 1,2-propanediol in the protein buffer (as a substitute for glycerol) to stabilize proteins and have seen improvement in the long term storage in many cases.
Hope this helps! annie Annie Hassell Glaxo Smithkline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) annie.m.hass...@gsk.com "LEDU Marie-Helene 161111" <marie-helene.l...@cea.fr> Sent by: "CCP4 bulletin board" <CCP4BB@JISCMAIL.AC.UK> 18-Nov-2009 03:45 Please respond to "LEDU Marie-Helene 161111" <marie-helene.l...@cea.fr> To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] trouble with protein storage Hello, Sorry for the unrelated question. We encounter important storage problem of our favorite protein. We can not freeze it without a lost of 50 to 90 %, and can not use glycerol without precipitation. Would anybody have suggestions or references that could help us ? Thanks a lot in advance. Marie-Helene Dr Marie-Hélène LeDu CEA/DSV/IBITEC-S/LBSR bat 144, CE Saclay 91191 Gif-sur-Yvette FRANCE tel 01 69 08 71 35 emel : marie-helene.l...@cea.fr
