Dear Muhammed,
that's not uncommon. Your protein might undergo domain movements or
contain flexible parts.
if your structure contains any loops / floppy regions (with high
B-values), you can exclude them from the search model.
If it is composed of domains, chop it into pieces and search with one
after the other, starting from the largest one.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Mon, 18 Jan 2010, Muhammed bashir Khan wrote:
Dear All;
We have solved a crystal structrure of protein at 1.8 A. I have now
another crystal of the same protein in aother unit cell,for the new
crystal type resolution is 3.6 A but when I use our structure as a seach
model It does't give any solution.
Any suggestion would be highly appreciated.
--
Muhammad Bashir Khan
Department for Biomolecular Structural Chemistry
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
Phone: +43(1)427752224
Fax: +43(1)42779522
