Dear All, Thanks to all who gave me good suggestions about my question regarding Buried surface area and oligomeric interface. I am posting the summary here.
---Biochemical Society Transactions (2008) Volume 36, part 6 (Kobe et al.,) ----have a look at this paper. I believe it's exactly what you're looking for. Protein-protein interaction and quaternary structure. <http://www.ncbi.nlm.nih.gov/pubmed/18812015> Janin J, Bahadur RP, Chakrabarti P. Q Rev Biophys. 2008 May;41(2):133-80. Review.PMID: 18812015 http://www.ncbi.nlm.nih.gov/pubmed/18812015 Paper: http://www.ncbi.nlm.nih.gov/pubmed/16923808 PISA: http://www.ebi.ac.uk/msd-srv/prot_int/cgi-bin/piserver ----Acta Cryst. (2007). D63, 1-8 -----There are a number of older papers that discuss the relationship between the conservation of residues and the possibility of an interface. I am assuming that this is a homodimer. Among them are: MaB. et al. 2003 PNAS 100:5772-5777. Valdar, W. and J Thornton. 2001 Proteins: Struct. Func. Gen. 42:108-124. Jones, S. and J Thornton. 1996 PNAS 93:13-20. You may also want to check the PISA server to see how they calculate the most favorable interfaces, or see what other structures show if it has been crystallized in a different space group. Thanks a lot Regards Yogi -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Sollepura Yogesha Sent: Monday, March 22, 2010 11:45 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Buried surface area (BSA) vs. oligomeric interface Dear All, We found that our structure is having a large buried surface in its dimer interface (~18% as calculated by AREAIMOL). This dimer has not been observed in earlier studies and right now we have less data to support this strong interface formation. I am looking for some literature which provide guidelines to understand the relation of BSA or crystal contacts with oligomeric interfaces. Any suggestion is highly appreciated Thanks in advance Yogi
