Dear Tim, I'd dare to say that SeMet labelling in mammalian cells is in fact routine (at least in our lab, and others' around the world) for quite a while :-) Since you mentioned CHO LecR cells, one can find references as early as 1994 from Wayne Hendrickson's lab (PMID: 7922031) and many others followed. More recently, people have moved to transient expression and probably the easiest labelling method for HEK293 cells can be found in PMID: 17001101.
Best wishes, radu ------------------------------------------ A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-287564 Fax: +44-1865-287547 ---- Original message ---- >Date: Tue, 23 Mar 2010 13:39:09 +0100 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Jovine Luca <luca.jov...@ki.se>) >Subject: Re: [ccp4bb] update on SeMet production >To: CCP4BB@JISCMAIL.AC.UK > >Dear Tim, > >Although it is not routine, it can be done in mammalian cells too! For example, have a look at this: > > http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.p df > >HTH, > >Luca > >------------------------------------------------------------- --- >Luca Jovine, Ph.D. >Karolinska Institutet >Department of Biosciences and Nutrition & Center for Biosciences >Hälsovägen 7, SE-141 57 Huddinge, Sweden >Voice: +46.(0)8.6083-301 FAX: +46.(0)8.6081-501 >E-mail: luca.jov...@ki.se >W3: http://jovinelab.org >------------------------------------------------------------- --- > >On Mar 23, 2010, at 1:25 PM, Tim Gruene wrote: > >> Dear all, >> >> since I am currenty preparing a lecture on crystallography I am wondering about >> the status quo of the production of SeMet proteins. >> In 2003, if I remember correctly, it was possible to express SeMet proteins in >> E.coli and insect cells. >> >> Has this been extended to other systems, and if so, which ones? >> >> Thanks a lot, >> Tim >> >> -- >> -- >> Tim Gruene >> Institut fuer anorganische Chemie >> Tammannstr. 4 >> D-37077 Goettingen >> >> GPG Key ID = A46BEE1A >>
