Hi, are you sure it is your protein precipitating? You will get a cloudy precipitate appearing in HEPES and Phosphate buffers on addition of ZnCl, without protein.
cheers charlie >>> dengzq1987 <dengzq1...@gmail.com> 3/31/2010 5:08 pm >>> hello everyone, recently i purify a protein conteining zinc binding domain,and i want to determine its structure.i get the crystal,but poor diffraction.so i try to adding zinc into the protein to optimize the crystal,but the protein precipitate immidiately even the znic is 1 mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does anyone have some advice? 2010-03-31 dengzq1987
