Hi, i'm not sure if you try just seeding in your original condition. If yes, you could try matrix seeding. Another option would be addtitves or the silver bullets from McPherson. Sometimes sitting drop vs. hanging drop make a difference. Mutations at the surface are quite often an excellent alternative to get new crystal forms.
Christian Am Mittwoch 07 April 2010 16:46:32 schrieb Debajyoti Dutta: > Hi All, > > I am dealing with a protein that crystallizes in 1D. The broom stick > crystals does not yield with any improvement w.r.t their dimension. I have > tried using different concentration of salt, ppt and pH around the parent > condition, even tried seeding and temperature changing. However, any of > these method does not help. > > Any suggestion is welcome. > > Thank you in advance. > > Sincerely > > Debajyoti >
