50% solvent content in a small crystal is still 50% solvent content in a large crystal given the same crystal form, so it's tough to use that to explain loss of diffraction.
Very large crystals in my hands seem to suffer from more problems of mechanical stress. William On Wed, Apr 14, 2010 at 5:36 PM, syed ibrahim <b_syed_ibra...@yahoo.com> wrote: > Hi All > > I had two crystals grown in same well, one is small and other is 10 times > bigger. I treated both crystal in same cryo and same time. The smaller one > diffracted to 2.5A and the bigger one to 6-7A. I was expecting the bigger one > to diffract high resolution. > > I assume the bigger crystal might have lot of solvent which prevent for high > resolution. If it is true what could be the best way to dehydrate crystal > without affecting crystal quality? > > Thank you > > Syed > > PS: Taken care of less solvent to be present in the loop > > > >
