On Thu, 2010-04-29 at 16:23 +0530, Jhon Thomas wrote: > This is a strange behaviour of the protein complex.
Why is this strange? 20 mg/ml is fairly high, just dilute the protein to 10 mg/ml and repeat the screen. Or better yet, repeat the screen with 1:2 protein:reservoir ratio for precipitated drops and 2:1 for clear ones. -- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /