Can you send your fragment of the pdb containing the ligand, and the cif
file?
Eleanor
Yahui Yan wrote:
Hello,
Could you please help me with the sketcher?
I'm trying to use ccp4 sketcher to generate a new ligand and then complex it
with a protein in coot. I've drawn the ligand, numbered each atom and
defined each bond type. Then I ran save file, create library description.
The pdb file was loaded to coot and worked fine. Then I imported cif file.
However, when I tried to refine the ligand, a message popped out, saying 'No
restrains'. I double checked the numbering, I think it's Ok. Did I do
anything wrong? I'm really struggling on this. If you need more information,
please let me know. Thanks very much.
Best regards,
Yahui
