Not quite sure if I understand you correctly, but if you mean with 'fix' to built a proper helix, then I would recommend to remove the helix first, run Refmac in Coot and than use the tool 'place helix here' using the difference density map to place the helix in density.
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Jul 16, 2010, at 8:56 PM, xinghua qin wrote: > Hi CCP4ers: > Recently I am trying to fix a helice, but found that it is so hard for me > because of the poor resolution(3.2A) and also a unvisible loop near it, so is > there any way to just fix a helice ? can I get it out of the whole structure > to refine it? > > Best regards > > -- > Xinghua Qin > College of Biological Sciences > No.2, Yuan Ming Yuan West Road > Haidian District，Beijing，China，100094 > Tel: +86-10-62732672
