I have a 30kDa protein which I have been trying to crystallize. I have tried in the conventional way using Hampton screens but no luck so far. CD of the protein shows it is folded. However reading the literature I found out it is strongly associated with the inner membrane of H. pylori. This was done by Western blots of the supernatant and membranes. Triton X-100 and N-lauroyl sarcosine were found to release the protein into the supernatant.
My question is do I change tactics and try and crystallize it as a membrane protein or try adding a small amount of detergents to the protein and treat it as a soluble protein? Or something else? Thanks in advance for all of your suggestions. Daniel Bonsor
