Interesting! it appears to be some kind of "secondary order"...I hope
someone wise/experienced can shed more light on this.
the diffraction spots appear to fall consistently in the middle of the
hexagonal(ish) grid lines, so it must be some partial order effect
related to the unit cell.
do you also see this with data collected from an unfrozen crystal? if
so, it is a crystal property.
you also appear to have quite strong solvent/ice rings, in any case I
would optimise the cryo-protection/freezing procedure.
Mark
Quoting xingliang zhang <xingliangche...@gmail.com>:
Dear everyone,
Recently,we collected data of a native protein crystal on synchrotron in
Shanghai. When we did with the original data with HKL2000, we found an
unconversant phenomenon ,just as the picture in the enclosure, there were
some grid indicated by arrows appearing on the low resolution areas.The
crytal grew in 20%PEG3000,100mMTris-Cl,200mM Ca(OAc)2 ,and the
cryo-protectant is 20% sucrose mixed with well buffer?Who can tell me the
reasons it appear the grid ?because of the sucrose? I'll appreciated for any
explains and suggestion.
Best wishes
xingliangzhang
--
Best wishes!
xingliangcheung,PhD,candidate
Protein crystallography Lab ,College of biological sciences ,China
agricultural university.
No. 2 yuanmingyuan west road HaidianDistrict, Beijing, 100193
Tel:01062814122,
E-mail:xingliangche...@gmail.com <e-mail%3axingliangche...@gmail.com>
