[FeS] clluster ? Or some metal bound to your protein ? Is it dark brown or yellowish brown ? What's your protein concentration ? > 25 mg/ml ?
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Sep 24, 2010, at 9:03 AM, Van Den Berg, Bert wrote: > Maybe you should give us a hint about the identity of your protein (if you > dare....;-)). I’m sure there are folks around who may be able to say whether > or not your protein is supposed to be brown. You can’t expect too much help > if you don’t provide (m)any details. > > Cheers, Bert > > > On 9/24/10 4:34 AM, "sandeep" <toskgu...@rediffmail.com> wrote: > > Dear all, > > I have purified protein from E.coli. expression system. the protein has been > purified with three independant columns. Now during concentration step using > amicon, the protein shows brown colour. what could be the reason. > > best regards and Thanks, > sandy > > <http://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/signatureline....@middle?> >
