In my hands GFP fusions can produce colorless crystals (but still have GFP!) depending on the condition. Sometimes the crystals gradually become colorless with time - bleaching or perhaps some chemical effect - no idea. I've not tested the colorless crystals with UV to see if they still glow.
Artem On Wed, Sep 29, 2010 at 3:41 AM, Tri Ngo <ngoduc...@gmail.com> wrote: > Dear CCP4 community, > > I wonder if anybody has experience about the color of GFP crystals. Is > there any chance that the GFP crystal will become colorless? We are working > on one membrane protein fused with GFP. Since we got some crystals which > didn't show any color (Condition 01), I am not sure if I should go for > optimization. > > Please check the link below for the image details: > http://www.docstoc.com/docs/55950590/GFP-crystals > > Because of the limited amount of sample, we used Topaz system (screening > chip) to do the screening and there is no way to confirm these crystals are > actually protein crystals. I noticed that the crystals are bigger day by > day. Is it a good sign to confirm the protein crystals? > > Thank you very much for your helpful information! > > Best wishes, > > * > TriNgo > > * > > Structural Biology Lab > > School of Medicine - Sungkyunkwan University > > Phone: 031-299-6150 > > >
