Dear ALL;
Firstly I'd like to thank folks for the various suggestions to handle the
degradation of MBP fusion protein.
According to these suggestions, I have tried shortening the linker between
MBP and target protein.
We can get decent yield of the full-length protein now.
We still have a little question about the expression.
Using BL21(De3 ) star cells at 37 degree, we got good soluble expression
of the MBP fusion protein. However, the majority of the proteins were existing
as soluble aggregates and only a small protion of proteins was purified as
monomers.
Does anyone have the experience to increase the yield of the monomeric
proteins?
Thanks a lot,
Jerry McCully
