It is somehow related to your second question. These folks describe a method for crystallization of low affinity complexes. I hope that helps.
Ignatev, A. et al. (2007) "A size filtration approach to purify low affinity complexes for crystallization" J. Str. Biol. 159(1), 154-157 Cheers, Mario Sanches On Wed, Nov 24, 2010 at 3:11 PM, Sollepura Yogesha <yoge...@scripps.edu>wrote: > Dear All, > > > > Could you please provide me some suggestions on following. > > > > 1. Could there be difference in interaction between two proteins when > produced > > a) both in bacteria, > > b) both IVTT (in vitro transcription and translation), and > > c) one in bacteria and other by IVTT. > > Could you please direct me to some references. > > > > 2. I am looking for references where two proteins not interacting by > pulldown or Native gel analysis (or other assays using purified protein) but > protein complex crystal structure was solved. > > > > Thanks > > Happy Thanksgiving > > Yogi > > > > > -- Mario Sanches Postdoctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave Toronto - Ontario Canada M5G 1X5 http://ca.linkedin.com/in/mariosanches
