Hi, Those are very high scores, which usually mean that the solution is correct.
One thing I'm wondering about -- when you run xtriage, does it report that you have translational non-crystallographic symmetry? Pseudo-translations can cause problems in molecular replacement. Other than that, I would suspect some sort of pseudosymmetry problem, or some other pathology with the data. Best wishes, Randy Read On 29 Nov 2010, at 13:16, xiuwen zhang wrote: > Dear colleages, > > Currently I met problem in a MR case and hope somebody could advise me. > > The crystals belong to sg C2221 with the cell parameters a=59A, b=302A, > c=97A, beta=92.3. The searching model shares 43% identities (300aa) with > target protein. When searching in PHASER program, only one clear solution > could be found with high Z-score: RZF=9.8 and TZF=24.2. However, though the > density is interpretative, the solution coordinate could not fit in the > density (Rfactor high to 54%). Moreover, as suggested by matthews_coff, there > should be at least 2 molecules in one ASU. But I could not find the other > molecules in the ASU in this case (though the other solution are defined with > high Z-score RZF=7.8 and TZF=59, this solution has 115 clashes with the first > one). I also find that this solution coordinate occupied some "layers" and > most of the unit cell are lack of density. As Phenix.xtrage suggested the > data is not twinned, I was totally confused by this situation. > > I will appreciate if you could share your opinions and experience on > similar case with me. > > Best Regards, > Xiuwen ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 Wellcome Trust/MRC Building Fax: + 44 1223 336827 Hills Road E-mail: rj...@cam.ac.uk Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
