Hi Kristof,
depending on what you termed "specific sites" the answer is definitely
yes.
I experienced such a case in the following reference related to the
AcrB structure but I remember that this situation arose also in the
case of the LTC4 structure.
-There is a baby in the bath water: AcrB contamination is a major
problem in membrane-protein crystallization.
Veesler D, Blangy S, Cambillau C, Sciara G.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):
880-5.
-Structural basis for synthesis of inflammatory mediators by human
leukotriene C4 synthase.
Martinez Molina D, Wetterholm A, Kohl A, McCarthy AA, Niegowski D,
Ohlson E, Hammarberg T, Eshaghi S, Haeggström JZ, Nordlund P.
Nature. 2007 Aug 2;448(7153):613-6.
All the best
David
Le 10 déc. 10 à 12:17, Kristof Van Hecke a écrit :
Dear,
I was wondering if anybody has experienced before the leakage of Ni-
ions (from a Ni-NTA column) and additionally binding to specific
sites in the protein structure..?
Many thanks
Regards
Kristof
--------------------------------------
Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
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