Dear Tom, There is a nice paper which addresses your problem. They coexpressed two representative Tyr kinases known for autophosphorylation activity with YopH Tyr phosphatase (Assuming yours is also a Tyr Kinase). You may contact the authors for the expression plasmid.
High yield bacterial expression of active c-Abl and c-Src tyrosine kinases (2005), SEELIGER et al., Protein Science (2005), 14:3135â3139. Good Luck!! Hari
