No - and I dont think it is accepted practice now either..
I often use I/SigI > 1.5 for refinement..
Look at your Rfactor plots from REFMAC - if they look reasonable at
higher resolution use the data
Eleanor
On 03/03/2011 11:29 AM, Roberto Battistutta wrote:
Dear all,
I got a reviewer comment that indicate the "need to refine the structures at an appropriate
resolution (I/sigmaI of>3.0), and re-submit the revised coordinate files to the PDB for
validation.". In the manuscript I present some crystal structures determined by molecular
replacement using the same protein in a different space group as search model. Does anyone know the
origin or the theoretical basis of this "I/sigmaI>3.0" rule for an appropriate resolution?
Thanks,
Bye,
Roberto.
Roberto Battistutta
Associate Professor
Department of Chemistry
University of Padua
via Marzolo 1, 35131 Padova - ITALY
tel. +39.049.8275265/67
fax. +39.049.8275239
roberto.battistu...@unipd.it
www.chimica.unipd.it/roberto.battistutta/
VIMM (Venetian Institute of Molecular Medicine)
via Orus 2, 35129 Padova - ITALY
tel. +39.049.7923236
fax +39.049.7923250
www.vimm.it
