Agree with Tim's idea. Generating a composite omit map to see if your solution is correct (or at least some part is correct if conformational difference exists between model and your protein).
Bingfa -----邮件原件----- 发件人: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk] 代 表 Tim Gruene 发送时间: 2011年4月27日 17:10 收件人: CCP4BB@JISCMAIL.AC.UK 主题: Re: [ccp4bb] A phase ambiguity problem. Dear Jashin, do you see any extra density in the map, of unexplained features? An easy and quick test is to remove some part of the search model, e.g. an alpha-helix, and recalculate the map. If you do see density for the missing helix, it is a very promising sign that your solution is correct. If you don't see anything chances are that it is a false solution, but at very low resolution, I would probably make further tests to be sure. Tim On Wed, Apr 27, 2011 at 04:28:49PM +0800, Jai-Shin Liu wrote: > Dear all: > > A phase ambiguity problem. > > After rotation/translation/refinement, the free value junk >0.5. > It seems no significant solution from rf file. INTERESTING, the density map seems to fit the template very well. > > In this case, is the phase still ambiguity or not? > > Thank you very much. Any comments will be appreciated. > > > Jashin > > The rf file of molrep is below: > theta phi chi alpha beta gamma Rf Rf/sigma Sol_RF 1 > 156.17 161.35 93.59 27.11 34.26 244.41 648.4 3.81 Sol_RF 2 163.22 > 179.18 121.99 29.25 29.26 210.90 561.2 3.30 Sol_RF 3 140.62 -179.55 > 61.93 65.56 38.11 244.67 556.5 3.27 Sol_RF 4 162.13 178.18 52.79 62.89 > 15.68 246.54 548.7 3.23 Sol_RF 5 136.93 -168.34 80.84 69.77 52.57 > 226.45 533.9 3.14 Sol_RF 6 165.86 -154.33 119.55 56.67 24.37 185.33 > 525.6 3.09 Sol_RF 7 148.21 162.97 129.01 12.27 56.79 226.33 522.2 3.07 > Sol_RF 8 165.70 -159.77 120.00 51.02 24.71 190.56 512.9 3.02 Sol_RF 9 > 164.58 -171.65 121.34 38.58 26.80 201.89 511.6 3.01 Sol_RF 10 144.59 > 159.55 61.67 43.61 34.56 264.50 503.4 2.96 -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
