You probably use hanging drops. It's the surface tension effect. Check
if sitting drops are better.
Maia
Sitting drops
weikai wrote:
Hi Folks,
We have some membrane protein crystals that are grown in 30%PEG400,
0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The
crystals are long rods and grown under room temperature in a hanging
drop set up. But once we open the cover slip, we see the rods start
to break and bend in a few seconds. Since it is in high PEG400, we
just directly freeze the crystals. The diffraction only goes to 10
Ang at synchrotron. Have anybody had similar problem before and any
suggestions?
Thanks a lot,
Weikai