Dear pengfei, you do not need to use mosflm if you want to scale the datasets with scala. You can use scalepack2mtz (available from ccp4i -> Program List) to convert the two sca-files to mtz-format and then use scala for scaling. Maybe HKL2000/scalepack can scale the files for you if you integrate them both in the same run (load both data sets, and HKL2000 will integrate both data sets).
Tim On Tue, May 31, 2011 at 02:40:49AM +0800, Pengfei Fang wrote: > Hi David, > > Thanks for your help. I just scaled the two data sets independently using > HKL2000. > And, I don't know how to set reference in HKL2000 or CCP4. > If using CCP4, should I also use imosflm to do integrate? > > thanks, > pengfei > > > At 2011-05-31 02:32:03，"David Veesler" <david.vees...@afmb.univ-mrs.fr> wrote: > Hi Pengfei, > you can combine your files using pointless but it is unclear to me based on > your message if you have scaled independently the two data sets or not. > You should scale these two data sets together using one as reference (doable > using XSCALE or SCALA...). > Cheers > David > > > Le 30 mai 2011 à 11:13, Pengfei Fang a écrit : > > Dear All, > > I have two data sets from one crystal. > One had short exposures to keep the low resolution reflections from > saturating. The other had long exposure to get high resolution data. > I am wondering how two merge these two data sets into one file that I can use > for refinement. > > The two sets already scaled by HKL2000. Now I have two .sca files. > > Thanks a lot! > Pengfei Fang > > > > -- > This message has been scanned for viruses and > dangerous content byMailScanner, and is > believed to be clean. > > > > -- > This message has been scanned for viruses and > dangerous content byMailScanner, and is > believed to be clean. -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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