Hi,
Thanks for all the comments, i was more wondering what the state of
the art might
be here, we have done similar thing at 4 Å (with 3 Å data though) 10
years or so back
with SnB. Maybe we need to get a heavier atom derivative indeed the
break the phases,
but i'll keep banging for now..
I have tried quite a lot, and was mainly wondering what was the world
record
nowadays in desperate solutions by SHELX/other direct methods...
playing around with XDS, data is finally quite ok to 6 Å low res anom
CC 100-90%,
SigAno about 3... goes down obviously but not terribly fast there is
signal all the way to 6Å.
Inverse beam data collection would probably be a good idea indeed next
time, native or
second wavelength or rahter both might help i assume..
thanks to Tim, Clemens, Poul and others for comments,
Best,
Tommi
On 15.6.2011, at 17.16, Tim Gruene wrote:
Hi Tommi,
Give it a try?
You give very little information, e.g.
- the data quality,
- possible radiation damage,
- isomorphism with a native data set, and
- what you have tried so far.
If you have a native data set with an isomorphous unit cell, try
SIRAS instead
of SAD.
Tim
On Wed, Jun 15, 2011 at 04:36:59PM +0300, tommi kajander wrote:
Dear all,
Does anyone have suggestions for 6 Å resolution phasing with large
number (40-50) Se sites (SAD so far)??
Thanks a bunch,
Tommi
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
