On 07/04/2011 04:24 PM, ruheng wrote:
>
> Dear all,
>
> Recently we are working on an archaebacteria protein which was expressed and
> purified from E.coli by conventional procedures. After we solved the
> structure, we found that there is an extra density in one of the argninine as
> shown in the attached picture. It seems that the density is larger than a
> methyl group and the atoms in the density are not on one plane. So we are
> curious about whether the density is a kind of posttranslational modification
> of arginine residues in the protein, if it is, what kind of modification
> could it be? And most important, what is the biological significance of this
> kind modification? Any suggestions and dicussions are appreciated!
>
> Best,
> Heng
>
We saw something like this and modelled it as glycerol. But I dont know
how one knows if one is correct!!
Eleanor
