This seems somewhat weird - your Rmerge values increase with more frames as explained by Tim, but I cant see why the I/SigI should increase sharply for the 180 degree set then fall off again. if you have run Scala look at the scaling plots v batch, and resolution to see if there are any weird outliers. Eleanor
On Mon, 11 Jul 2011 10:23:00 +0200, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > Dear Vennila, > > my guess is that you had a larger number of images in the batch/set > rge starting at > 180 degrees (hence the slightly incereased Rmerge for that batch) and that > by > the end of that run your crystal had suffered from radiation damage (hence > the > large Rmerge for the set starting at 360degrees). > > You should report Rmeas instead of Rmerge and let us know about the number > of > frames and the frame width in each set. > > Cheers, Tim > > > On Sun, Jul 10, 2011 at 01:54:07PM +0100, Vennila Natesan wrote: >> Dear CCP4BB users, >> >> In order to determine the redundancy at which the structure can be >> solved, I divided the master >> data set of my protein into five sets with phi rotations of 45,65,90, >> 180 and 360 degrees. I got >> the mean I/Sigma value as 22.4(11.4), 21.7(11.0), 22.7(11.2), 60.9(45.2) >> and 15.6(8.1) respectively. >> I will be very helpful if i get any idea/possible reasons for the >> abnormal value for 180 degree dataset. >> >> For the information, the completeness values are >> 75.7(78.5),92.6(92.7),99.5(97.7),99.6(97.7)and 99.5(96.4) >> respectively and values inside brackets are for highest resolution >> shell. There was no noticable change in mosaicity >> value. The Rmerge values are 2.2, 2.4, 2.7, 3.2, and 5.7 (8.7) >> respectively. >> >> Thanks in advance >>
