Hi Ivan, Here is another example of a method to crystallize antibody/antigene complexes. It uses a limited proteolysis step to generate crystals of poor quality, which are then used as seeds for an MMS screening...
http://www.ncbi.nlm.nih.gov/pubmed/21536542 Good luck, Alex 2011/7/28 xaravich ivan <xaravich.i...@gmail.com> > Hi everyone, > I have been trying to crystallize Fab:antigen complex( 50kda:90kDa) complex > and initially got needle clusters which after microseeding gave me single > crystals but they are very small and I could not repeat the results. I have > been using HEPES buffer at pH 6.8 to do the final SEC purification step of > the complex before setting trays. > I was wondering whether there are some other buffers (that one could > suggest eg tris-hcl etc) which have given decent positive results when > crystallizing Fab complexes.Though I have gone through individual papers > (case by case) to get some idea, It would be great if anyone could direct me > to a comprehensive literature towards studying the crystatllization > conditions of Fab complexes. > Equally, people who have considerable experience could suggest a list of > must do steps for such problems which have routinely been practiced in their > lab > > > Also what is a good storage condition for the excess complex that you want > to use later? > > I would really appreciate any suggestion,help, direction. > > Thanks > ivan >
