You should check these "spherulites" on SDS-PAGE gel to make sure that these contain your protein. Then you can start thinking about optimization.
On Wed, Aug 24, 2011 at 2:05 PM, Jan van Agthoven <janc...@gmail.com> wrote: > Dear all, > > I recently obtained some spherulites while trying to crystallize my > protein. The spherulites are manually reproducible, but changing pH, protein > concentration, and salt concentration does not result in crystal formation. > Microseeding with crushed spherulites isn't a solution either as it only > yields new spherulites. Next stepp is the use of an optimization kit but I > have a limited amount of material, and I start doubting that these are > protein spherulites, as the spherulites are not particularly soft. The > condition contains 15% PEG 3350 and 200 mM NaCl. Does anyone know if PEG > 3350 forms easily spherulites around that concentration? > > > Thanks, >
